EFFECT OF ARTIFICIAL MITOCHONDRIAL TRANSFER FROM LINE CELLULAR HSC-3 TO LYMPHOCYTE TCD4+, AS A MECHANISM INDUCTOR OF EXHAUSTED PHENOTYPE.

Abstract Background: Oral squamous cell carcinoma (OSCC) is the most frequent type of oral cancer. The tumor microenvironment OSCC induces an alteration T lymphocyte, promoting exhausted phenotype. main organelle metabolism mitochondrion and in recent years it has been indicated that several cells have capacity to transfer mitochondria, including cancer cells. However, not evaluated whether mitochondria from lymphocytes phenotype lymphocyte. aim this work was analyze TCD4+ after artificial (MitoCeption) obtained line HSC-3. Methods: Isolated MitoTracker-labeled were Mitocepted into CD4+ lymphocytes. Then, surface molecule expression, proliferation cytokine secretion mediated by mitochondrial analyzed flow cytometry. Results: results showed acquired had increased expression 2 inhibitory proteins (TIGIT CTLA4) 3 associated with (PD-1, PLD-1 LAG3), compared control group. In addition, mitocepted exhibited a significant decrease control. For analysis, observed group IFN-gamma, TNF-alpha, IL-10 IL-4, but IL-17, Conclusions: Therefore, we concluded acquisition isolated HSC-3 lymphocyte Regular Fondecyt Project 1211480